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. 2016 Feb 17;6(4):558–570. doi: 10.7150/thno.14693

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Down-regulation of Hsp27 attenuates HCC cell migration and invasion. (A-B) The detection of shRNA-mediated knockdown of Hsp27 in MHCC97H and HCCLM3 cells by real-time PCR and western blotting. (C) A representative result of the CCK-8 assays for the effects of Hsp27 silence on proliferation of MHCC97H and HCCLM3 cells. (D) Transwell assays for the effects of Hsp27 silence on migratory and invasive potentials of MHCC97H and HCCLM3 cells (magnification, ×100). (E) Representative images for metastatic nodules in indicated groups (magnification, ×100, ×200). (F) Quantification of metastatic nodules in control and Hsp27-silenced groups. **P < 0.01, ***P < 0.001.