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. 2016 Mar 2;94(3):626–633. doi: 10.4269/ajtmh.15-0452

Figure 3.

Figure 3.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot analysis of recombinant annexin B33 protein of Echinococcus granulosus (rEg-ANX). Expression, purification, and identification of rEg-ANX by serum of E. granulosus-infected mice. M = molecular mass marker in kDa; lane 1 = whole cell protein from isopropyl-β-d-1-thiogalactopyranoside–induced Escherichia coli cells containing pET-32a-Eg-ANX; lane 2 = sample after Ni column purification of recombinant protein; lane 3 = purified rEg-ANX was probed with the serum of E. granulosus infected mice; lane 4 = purified rEg-ANX was probed with native mouse serum.