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. 2016 Mar 2;94(3):626–633. doi: 10.4269/ajtmh.15-0452

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Figure 4. — Transcription and expression of endogenous annexin B33 protein of Echinococcus granulosus (Eg-ANX) in E. granulosus. (A) Identification of endogenous Eg-ANX from E. granulosus. M = molecular mass marker in kDa; lane 1 = purified rEg-ANX was probed with anti-rEg-ANX rabbit serum; lane 2 = purified rEg-ANX was probed with native rabbit serum; lane 3 = the anti-rEg-ANX rabbit serum detected a single band at a mass of 37 kDa in E. granulosus cystic fluid; lane 4 = the cystic fluid was probed with native rabbit serum; lane 5 = endogenous Eg-ANX from PSCs was probed with anti-rEg-ANX rabbit serum; lane 6 = the total protein of PSCs was probed with native rabbit serum; lane 7 = the total extracts of healthy sheep was probed with anti-rEg-ANX rabbit serum. (B) Endogenous Eg-ANX transcription of E. granulosus metacestode determined by quantitative real-time polymerase chain reaction. The transcripts of Eg-ANX in laminated layer were not detected. The elongation factor 1 alpha was used as the transcriptional control, and the data were presented as mean ± SD. GL = germinal layer; PSCs = protoscoleces.