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Parasite dynamics by quantitative real-time polymerase chain reaction (qPCR) after controlled human malaria infection (CHMI) #1. Individual parasite density curves of control subjects (N = 5) measured by qPCR are shown up to day of treatment, based on diagnosis by thick smear. The gray dotted line indicates the average parasite detection limit for microscopy. Four of five subjects were first positive on day 7. One subject was first positive on day 10.5.
