TABLE 2.

In vitro trypanocidal activities of FTY720, sphingolipids, and SPHKI against T. brucei subspecies

Compound	IC50 (mean ± SD) (μM) fora:				SI HEK293b
	T. brucei brucei	T. brucei rhodesiense	T. brucei gambiense STIB930	T. brucei gambiense K048
FTY720	0.59 ± 0.01	0.20 ± 0.01	0.01 ± 0.01	0.072 ± 0.08	16.8
FTY720P(S)	1.21 ± 0.07	0.71 ± 0.09	0.03 ± 0.01	0.40 ± 0.13	14.8
FTY720P(R)	1.98 ± 0.26	1.06 ± 0.21	0.11 ± 0.05	0.67 ± 0.05	9
Sphingosine	1.26 ± 0.35	0.63 ± 0.09	0.54 ± 0.02	0.53 ± 0.08	31.4
d-Erythro-sphinganine	1.71 ± 0.05	0.99 ± 0.04	0.86 ± 0.04	0.72 ± 0.08	6
d-Methyl-sphingosine	1.63 ± 0.12	0.58 ± 0.11	0.02 ± 0.02	0.18 ± 0.01	12.2
N-hexanoyl-d-sphingosine	2.89 ± 0.83	3.03 ± 0.17	6.17 ± 0.91	4.25 ± 0.43	1.4
CAY10621	0.48 ± 0.36	0.19 ± 0.03	0.06 ± 0.06	0.09 ± 0.14	20.8
PF543	10.79 ± 3.07	3.10 ± 1.25	0.51 ± 0.14	2.13 ± 0.16	1.8
SKI2	1.95 ± 0.92	3.73 ± 0.09	0.20 ± 0.03	0.34 ± 0.27	20.3
Pentamidine	0.0027 ± 0.0007		0.0007 ± 0.0002	0.051 ± 0.016	260.8
Diminazene aceturate	0.027 ± 0.001				957
Puromycin	0.038 ± 0.002				12
Melarsoprol		0.007 ± 0.003	0.0006 ± 0.002	0.014 ± 0.002

^aResults from three independent experiments.

^bSelectivity index (SI) compared to HEK293 cells.