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MICs of fluconazole, obtained by Etest, for GRT78 strains. (A and D) Wild-type GRT78 strains with fluconazole MICs of 32 μg/ml. (B) mdr1Δ mutant strain (MIC, 32 μg/ml). (C) MDR1 reconstituted strain (mdr1::MDR1) (MIC, 32 μg/ml). (E) pdr11Δ mutant strain, for which the fluconazole MIC was significantly reduced, to 0.06 μg/ml. (F) PDR11 reconstituted strain (pdr11::PDR11), with reversion to wild-type fluconazole susceptibility (MIC, 32 μg/ml).
