Phylogenetic analysis of Tn5801 variants in Firmicutes. The 4 numbers separated by lines indicate the four core fragments (intTn5801, orf18-14, orf12-8, and orf7-4) representing the common region in all Tn5801 variants described in the GenBank database. These sequences were aligned, concatenated, trimmed, and further analyzed. A maximum-likelihood phylogenetic tree is represented, with 1,000 bootstrap replications. The orf26-19 fragment was excluded from this analysis because of its variability (resulting from both mutation and recombination events). The distribution of Tn5801 variants by species and geographical regions is also represented. Countries within each continent are represented by different tones of gray, black and white. Sau, Staphylococcus aureus; Sps, Staphylococcus pseudintermedius; Efm, Enterococcus faecium; Efs, Enterococcus faecalis; Evi, Enterococcus villorum; Sag, Streptococcus agalactiae, Smi, Streptococcus mitis, Sth, Streptococcus thoraltensis; Lga, Lactococcus garvieae; ARG, Argentina; AUS, Australia; BEL, Belgium; BRA, Brazil; CHE, Switzerland; CHN, China; DEU, Germany; DNK, Denmark; FRA, France; GBR, Great Britain; HKG, Hong Kong; IRL, Ireland; JPN, Japan; MYS, Malaysia; NLD, The Netherlands; PRT, Portugal; ESP, Spain; TWN, Taiwan; USA, United States; unk, unknown.