TABLE 1.

Characterization of Tn5801: PCR strategy and conditions

Primer	Sequence (5′→3′)	Position in Tn5801 (bp)	PCR	Sequence amplified	Amplicon size (bp)	Amplification conditions
gmp1	CCTGGTCTTGGTATTCGTGT	25763–25783a	gmp1-gmp2	guaA	170	1 cycle of 95°C for 10 min; 25 cycles of 94°C for 30 s, 54°C for 30 s, 72°C for 30 s; 1 cycle of 72°C for 10 min
gmp2	GTAGTCTCCCATAACACC	25609–25627a
Int1	CTGTTTCCGATATTGAGC	24490–24473a	Int1-gmp1	intTn5801-guaA	1,311	35 cycles of 96°C for 30 s, 45–59°C (according to primer pair) for 1 min, 72°C for 7 min; 1 cycle of 72°C for 10 min
P1	GTTTCGCAAGTAGTCTACAG	25310–25329a	P1-P2	orf25-intTn5801	2,085
P2	GTAAAGGCGACAGATGG	23244–23260a
P3	CTTAGAGATGAGTTTCGTTTC	23391–23411a	P3-P4	orf22-orf25	1,455
P4	GGATAGTTCTTTGTCTGTAAAG	21956–21977a
P5	CGATTTTAGAGCCGTTGGTTTAG	22210–22232a	P5-P6	tet(M)-orf22	2,448
P6	GAACGTCAGAGAGGAATTAC	19784–19803a
P7	GGGAATCCCCATTTTCCTAA	19986–20005a	P7-P8	orf15-tet(M)	6,498
P8	ACCCACTCGCTGTTTAATCG	13507–13526a
P9	CTTGCAAGGCTAGGTTGGAG	14162–14181a	P9-P10	orf7-orf15	5,993
P10	ATGTCTGAAATGGGCTTTGG	8188–8207a
P11	CTTTCACTTCGTGCGGTACA	8382–8401a	P11-P12	orf3-orf7	4,631
P12	GGGTGGGGACAATACATCAG	3770–3789a
P13	CAGCCATGTAGCGTCTTTGA	4315–4334a	P13-P14	orf1-orf3	4,228
P14	ACGGAGTTAACGGCTTTCCT	106–125a
P15	CCGTATGTTCTTTCAACCACT	1030–1051b	P15-P11	orf5-orf7	2,675
P16	GCTGACAGTTCCAGTATCC	18096–18115c	P16-P5	orf23′-orf22	1,209
P17	CAGAACCAGCCATTACC	18561–18578c	P17-P18	orf23′-orf19′	3,938
P18	GAAGATACGAGAAACCAATAG	14602–14623c
P19	GGTGGCAATTCAAGTGTTCC	14859–14878c	P19-P20	orf19′-orf17	1,957
P20	GGCGTATGACAAAGCTGG	12921–12939c

^aReference sequence, Staphylococcus aureus Mu50 (GenBank accession number BA000017).

^bReference sequence, Streptococcus agalactiae COH1 (GenBank accession number AAJR01000021.1).

^cReference sequence, Enterococcus faecalis JH2-2 (GenBank accession number AXOI01000011.1).