TABLE 4.

Results of filter mating assays with Enterococcus faecalis strains^a

Transconjugant	Donorb	Recipient	No. of Tn5801 copies detected	Hybridized SmaI-digested DNA band size (added fragment size), kb	Hybridized probe			Transfer rate per donor	Growth rate, mean/SD	Relative growth rate, % (transconjugant/recipientc)	Tet MIC, μg/ml
					int	tet	gua
JH2-2::Tn5801_1	E. faecalis Ef1	JH2-2	1	200 (50)	+	+	+	1.6 × 10−8	0.15437/0.01096	3.66385	48
JH2-2::Tn5801_2	E. faecalis Ef1	JH2-2	2	250 (100)	+	+	+	1.6 × 10−8	0.15489/0.01568	3.34281	96
OG1SS::Tn5801_1.1	JH2-2::Tn5801_1	OG1SS	1	150 (20)	+	+	+	1.81 × 10−7	0.16787/0.00576	3.16674	32
OG1SS::Tn5801_1.2	JH2-2::Tn5801_1	OG1SS	2	340 (20)	+	+	−	1.01 × 10−8	0.16773/0.00321	3.24749	96
				150 (20)	+	+	+
OG1RF::Tn5801_1	E. faecalisEf1	OG1RF	1	150 (20)	+	+	+	3.3 × 10−8	0.16087/0.00769	0.16068	24
OG1RF::Tn5801_2	E. faecalis Ef1	OG1RF	1	150 (20)	+	+	+	3.3 × 10−8	NDd	ND	48
OG1RF::Tn5801_3	E. faecalis Ef1	OG1RF	1	150 (20)	+	+	+	3.3 × 10−8	ND	ND	64
OG1SS::Tn5801_2.1	JH2-2::Tn5801_2	OG1SS	1	150 (20)	+	+	+	2 × 10−7	0.17217/0.00709	0.68706	96
OG1SS::Tn5801_2.2	JH2-2::Tn5801_2	OG1SS	2	150 (20)	+	+	+	2 × 10−7	0.17717/0.00661	−2.19576	96
				120 (20)	+	+	−
OG1SS::Tn5801_2.3	JH2-2::Tn5801_2	OG1SS	2	200 (50)	+	+	−	1.6 × 10−5	0.17283/0.00776	0.30828	96
				170 (50)	+	+	+
OG1SS::Tn5801_2.4	JH2-2::Tn5801_2	OG1SS	1	150 (20)	+	+	+	1.6 × 10−5	0.16903/0.00645	2.50019	32

^aFollowing the order presented in Fig. S3 in the supplemental material.

^bEnterococcus faecalis Ef1 tetracycline MIC, 48 μg/ml.

^cRecipient growth rates (means/standard deviations): 0.16024/0.01179 (E. faecalis JH2-2), 0.17336/0.00362 (E. faecalis OG1SS), and 0.16112/0.00771 (E. faecalis OG1RF).

^dND, not determined.