TABLE 4.

Mutations found in resistance-conferring regions of gyrA and parC in the wild-type strains and deletion mutants during acquisition of resistance to enrofloxacin in mineral medium^a

Enrofloxacin concn (μg/ml)	Mutations(s) found in the following region of the indicated strain:
	gyrA									parC
	BW25113	MG1655		ΔsoxS mutant		ΔgadE mutant		ΔompF mutant		BW25113	MG1655		ΔsoxS mutant		ΔgadE mutant		ΔompF mutant
		1	2	1	2	1	2	1	2		1	2	1	2	1	2	1	2
0
0.125	D87G	S83L	S83L		S83L	D87G	D87Y	D87G	S83L
0.25	S83L	S83L	S83L, D87N	D87Y	S83L	G81C, R38R*	S83L, D87Y*	D87G	S83L
0.5	S83L, D87N	S83L	S83L, D87N	G81D	S83L, D87G	G81C	S83L	D87G, R38R*	S83L, D87G
16	S83L, D87N	S83L, D87G	S83L, D87N	G81D	S83L, D87G	G81D	S83L	S83L, D87G	S83L, D87G	S80I, E84V	S80I	S80I, E84V	S80I		S80R	S80I		S80I
1,024	S83L, D87N, H91T	S83L, D87G	S83L, D87N	G81D	S83L, D87G	G81D	S83L, D87N	S83L, D87G	S83L, D87G	S80I, E84V	S80I	S80I, E84V	S80I, D79A	S80I	S80R	S80I	E84G	G78D

^aResults are for two independent replicates (1 and 2) except for the BW25113 wild type, for which the results are for only one assay. *, only one out of two colonies carried the mutation.