TABLE 4.
Enrofloxacin concn (μg/ml) | Mutations(s) found in the following region of the indicated strain: |
|||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
gyrA |
parC |
|||||||||||||||||
BW25113 | MG1655 |
ΔsoxS mutant |
ΔgadE mutant |
ΔompF mutant |
BW25113 | MG1655 |
ΔsoxS mutant |
ΔgadE mutant |
ΔompF mutant |
|||||||||
1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | |||
0 | ||||||||||||||||||
0.125 | D87G | S83L | S83L | S83L | D87G | D87Y | D87G | S83L | ||||||||||
0.25 | S83L | S83L | S83L, D87N | D87Y | S83L | G81C, R38R* | S83L, D87Y* | D87G | S83L | |||||||||
0.5 | S83L, D87N | S83L | S83L, D87N | G81D | S83L, D87G | G81C | S83L | D87G, R38R* | S83L, D87G | |||||||||
16 | S83L, D87N | S83L, D87G | S83L, D87N | G81D | S83L, D87G | G81D | S83L | S83L, D87G | S83L, D87G | S80I, E84V | S80I | S80I, E84V | S80I | S80R | S80I | S80I | ||
1,024 | S83L, D87N, H91T | S83L, D87G | S83L, D87N | G81D | S83L, D87G | G81D | S83L, D87N | S83L, D87G | S83L, D87G | S80I, E84V | S80I | S80I, E84V | S80I, D79A | S80I | S80R | S80I | E84G | G78D |
Results are for two independent replicates (1 and 2) except for the BW25113 wild type, for which the results are for only one assay. *, only one out of two colonies carried the mutation.