. 2016 Feb 26;60(3):1319–1327. doi: 10.1128/AAC.02684-15

TABLE 6.

Mutations found in resistance-conferring regions of gyrA and parC in wild-type cells adapted to enrofloxacin in mineral medium under different conditions^a

Enrofloxacin concn (μg/ml)	Mutations(s) found in the following region of the indicated strain under the indicated condition:
	gyrA						parC
	BW25113, pH 6	MG1655, pH 6		BW25113, 2% salt gyrA	MG1655, 2% salt gyrA1		BW25113, pH 6	MG1655, pH 6 parC	BW25113, 2% salt parC	MG1655 2% salt, parC1
	BW25113, pH 6	1	2	BW25113, 2% salt gyrA	1	2	BW25113, pH 6	MG1655, pH 6 parC	BW25113, 2% salt parC	1	2
0
0.125						S83 deletion^b
0.25	G81D	D87G	D87N		D87T
0.5	G81D		D87N	D87N
16	D87N	S83W	D87N	D87G	S83L	D87G			S80I		S80R
1,024	G81D, D87N	G81D	D87N	D87N	S83L, D87G	S83L, D87G	E84G	S80R	G78D	E84G	S80I

Results are for two independent biological replicates (1 and 2) for all strains except the BW25113 strain, for which the results are for only one assay.

The S83 deletion was observed in only one out of two colonies.