FIG 3.

Intranasal inoculation of mice with C646 significantly suppresses viral replication in the lungs and improves survival following influenza virus infection. (A) BALB/c mice were intranasally inoculated with C646 or DMSO as shown in the schematic diagram. (B) Mice were then infected with 5 × 10³ PFU of the WSN virus. On days 3 and 6 postinfection, three mice from each group were euthanized, and lung samples were collected and titrated for virus in MDCK cells. *, P < 0.05 (Student's t test). Mouse body weight (C) and survival (D) were monitored for 14 days postinfection. DMSO-I, mice were inoculated with DMSO and also infected with virus; 1 mg/kg-I, mice were inoculated with 1 mg/kg C646 and also infected with virus; 5 mg/kg-I, mice were inoculated with 5 mg/kg C646 and also infected with virus; DMSO, mice were inoculated with just DMSO and not infected with virus; 5 mg/kg, mice were inoculated with just 5 mg/kg C646 and not infected with virus. Data are presented as means ± SD (n = 6); the P value is based on the Mantel-Cox test.