FIG 1.

Porin loss does not significantly alter growth, cellular integrity, or the rate of OMV production. (A) Growth curves of Klebsiella pneumoniae clonally related isolates with distinct expression patterns of Ompk35 and OmpK36. (B) Western blot of cytoplasmic RecA in whole-cell lysate (WCL) and concentrated culture supernatant (Sup.) to detect cell lysis or leakage due to changes in membrane composition. (C) Outer membrane vesicle production as determined by total phospholipid assay. Culture supernatant phospholipid content was assayed and normalized to the culture CFU. n = 3. Bars show means and standard deviations from the means.