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(A–F) FRET efficiency histograms determined by confocal microscopy for (A) free IκBα labeled at 166 and 262 (AR3–6), (B) IκBα (AR3–6) bound to dimerization-domain-only NFκB, (C) IκBα (AR3–6) bound to full-length NFκB, (D) Free IκBα labeled at 128 and 262 (AR2–6), (E) IκBα (AR2–6) bound to dimerization-domain-only NFκB, and (F) IκBα (AR2–6) bound to full-length NFκB.
