Table 1.

Nontraditional Events Were Characterized by Their 10/90 Slope and Area

Name	10/90 Slope	Area (fC)	Percent Release	Release Mode
Kiss-and-Run	<6	<100	0–20	small release
Partial Fusion	<6	100 < area < 385	20–80	partial release
Full Fusion	<6	385 < area	80–200	full release
Bulk Fusion	>6	960 < area	>200	OCS or combining granule release

As NTEs are often characterized by slower pore opening, the 10/90 slope was determined to be a good indicator of NTEs. To distinguish between the different types of NTEs the extent of release, or area under the current spike, was used. Commonly, events releasing greater than twice the amount of expected serotonin, had steep 10/90 slopes that were most likely due to more serotonin occupying the larger free space outside the dense-body core. Therefore, bulk fusion events had a 10/90 slope > 6. All slopes and areas were calculated using Mini Analysis software.