Table 1.

Numbers of Counted Points and Average Localization Precision per Image: 10 nm GNPs

Cell Number	Counts of GNP Points (2 h)	Counts of GNP Points (18 h)	Localization Precision (2 h) (nm)	Localization Precision (18 h) (nm)
1	1230	12,498	14.44	20.73
2	28,245	14,379	18.87	21.27
3	13,649	13,711	19.32	21.07
4	1762	6150	5.87	22.25
5	25,055	9699	19.03	22.27
6	14,754	8519	20.00	22.12
7	11,877	8995	20.38	21.52
8	12,563	8118	20.86	22.33
9	14,414	3597	18.79	21.53
10	7285	12,703	19.80	21.84
11	1438	10,362	17.57	21.61
12	4491	8658	34.94a	20.22
13	4262	8242	35.83a	21.75
14	20,404	9374	31.67a	22.51
15	7632	5908	33.53a	20.97
Average	11,271	9394	17.20	21.60
SD	8466	2998	8.33	0.66
Error of the mean	2263	801	2.23	0.18

The GNPs were incubated for 2 and 18 h and illuminated at 561 nm.

^aObtained with a slightly differently positioned localization detection lens. This resulted in a lower amount of signals, so the threshold of the analysis was adjusted. This means that the amount of counted points is consistent with the rest of the measurements, but the localization precision was poorly affected.