Fig. 3.

BCP determination of daptomycin susceptibility. (a) Daptomycin non-susceptible (DNS; gs5) and daptomycin susceptible (DS; ATCC29213) cell walls were stained with WGA-Cy5 (red) while the DNA was stained with DAPI (blue) and SYTOX Green (green). Images were taken after treatment with no antibiotics or 5 μg/mL of daptomycin for 30 min. (b) LDA plots were calculated from cytological profiles after 30 min of treatment with daptomycin where parameters were the mean of triplicates. The LDA plot consists of the mean cytological profiles 8 DNS strains (red) and 12 DS strains (blue), all of which forms the training set. Blind test profiles (filled circles; n = 12) are overlaid on the training set.