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. 2016 Mar 3;6:22460. doi: 10.1038/srep22460

Figure 4. APP overexpression inhibits cell proliferation and the downregulation of two identified genes is involved in proliferation impairment.

Figure 4

(A) Growth curve of HEK293 cells and HAW cells. Equal number of cells was seeded on 35 mm dishes and the cell number was counted every 3 days. The growth curve was plotted after 12 day’s culture. Values represent mean ± SEM. N = 3, *p < 0.05 by ANOVA. (B) BrdU cell proliferation assay of HEK293 and HAW cells. After 6-hour incubation with BrdU, BrdU incorporation was detected by BrdU antibody followed by cy3-conjugated anti-goat IgG (Red). Nuclei were stained with DAPI (Blue). (C) Quantification of BrdU positive cells in HEK293 and HAW cells. The percentage of BrdU-positive cells in each line was presented. Values represent mean ± SEM. N > = 3, *p < 0.05 by student t-test. (D) 3 days after scramble, PSMA5 or PSMB7siRNA transfection, the expression of PSMA5 or PSMB7 was examined by Wstern blot. PSMA5 or PSMB7 was detected by PSMA5 or PSMB7 antibody. β-actin served as internal control was detected by mouse anti-actin antibody. (E) 3 days after scramble, PSMA5 or PSMB7siRNA transfection, BrdU cell proliferation assay was performed. BrdU incorporation was detected by BrdU antibody followed by cy3-conjugated anti-goat IgG (Red). Nuclei were stained with DAPI (Blue). (F) Quantification of BrdU positive cells. The percentage of BrdU-positive cells in each line was presented. Values represent mean ± SEM. N > = 3, *p < 0.05 by student t-test.