Figure 2. Detection of major capsid proteins.

HEK293 cells were transfected with pAd5, pAd5-37(5, 7), pAd5-43(5, 7) pAd5-37(1–7) and pAd5-43(1–7) genomic plasmids. Cells were harvested at the indicated time points. (a) Total cell extracts were prepared, separated by SDS-PAGE and subjected to immunoblotting using anti-penton, anti-fiber or anti-hexon rabbit polyclonal sera and mouse tubulin mAb as a loading control. (b) Total-cell extracts were prepared, and hexon trimers were separated by native-PAGE and subjected to immunoblotting using anti-hexon goat pAbs from serotype 5 and serotype 37, and a mouse tubulin mAb was used as a loading control. All Western blotting experiments were repeated three times.