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. 2016 Mar 3;6:22464. doi: 10.1038/srep22464

Figure 4. Chimeric hexon trimerization in presence of L4-100K protein.

Figure 4

Chimeric hexon plasmids with or without the L4-100K plasmid were co-transfected into HEK293 cells, which were harvested at the indicated times points. (a) Total cell extracts were prepared, hexon trimers were separated by native-PAGE and subjected to immunoblotting using anti-hexon goat pAbs, hexon monomers were separated by SDS-PAGE and subjected to immunoblotting using anti-hexon goat pAbs, Ad5 L4-100K protein was detected by immunoblotting using an anti-HA mouse mAb. (b) Serotype-specific response between Ad5 L4-100K, subgroup D L4-100K and 5-Hexon plasmids were investigated by co-transfection into HEK293 cells as indicated, and Western blotting was performed at 72 h. All co-transfection experiments were repeated three times.