Figure 5. Nuclear transfer of chimeric hexons in presence of L4-100K protein.

(a) Expression of variant hexons was observed in HEK293 cells transfected with 1 μg of each plasmid by confocal microscopy. At least 30 independent cells were examined in each sample, and the most representative cells are shown. Hexon-C: hexon in cytoplasm, Hexon-N: hexon transferred into nucleus. Blue: cell nucleus. Red: hexon protein. (b) The proportions of positive cells expressing hexon in the cytoplasm or transferred to the nucleus at 72 h post transfection were calculated.