Figure 5. Reduced GABA_A receptor agonist sensitivity of nigral dopamine neurons from eNRG1-pretreated mice.

(a) Inward currents were triggered by application of 1 mM GABA to midbrain slice preparations in the presence of the GABA_B receptor antagonist CGP52432. Typical traces are displayed. (b) The peak amplitudes are plotted and compared (n = 17 cells from 6 control mice and n = 23 cells from 8 eNRG1-pretreated mice). **p < 0.01 by Mann–Whitney U test. Note; in the presence of CGP52432, input resistance of eNRG1-pretreated cells was rather larger than control (control: 252 ± 18 MΩ; eNRG1: 391 ± 32 MΩ, p < 0.01), but no differences were detected in other electrophysiological properties (data not shown). The GABA_A receptor blocker PTX markedly reduced the GABA-evoked currents (see Supplemental Information Fig. S1).