Figure 6. Effects of perinatal eNRG1 pretreatment on I_AHP in adult dopaminergic neurons.

(a,b) Unclamped action potentials were elicited in nigral dopamine neurons of control and eNRG1-pretreated mice. Depolarizing pulses (duration 2 ms, marked with arrows) from a holding potential of −55 mV to +40 mV were given. The peak amplitude (a) and total charge transfer (b) of the outward tail current were measured (n = 22 cells from 9 control mice, n = 30 cells from 12 eNRG1-pretreated mice). The peak amplitude was not affected (662 ± 56 pA for control, 727 ± 33 pA for eNRG1, p = 0.30, Mann-Whitney U test), but the total charge transfer was marginally decreased in eNRG1-pretreated mice (10773 ± 816 pA•ms for control, 8978 ± 647 pA•ms for eNRG1, (*) p = 0.066, Mann-Whitney U test).