Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Strategy for the construction of SAFV and chimeric SAFV constructs. The pJET-SAFV plasmid was generated by insertion of SAFV cDNA amplicon into pJET-hPolI/mTer. Further, DA L gene from TMEV was flanked with linear pJET-SAFV between 5'UTR and VP4 using an In-Fusion cloning method. After transfection of pJET-DAL-SAFV into Vero cells, chimeric SAFV virus was rescued. PI: human polymerase I promoter; 25: poly (A) tail with 25 As; mT: murine terminator.
