Figure 3.

Vimentin-specific IgM and MOG-specific IgG autoantibody titers during acute IM following normalization to serum total IgM and serum total IgG. Total IgM and IgG (A) concentrations were measured in serum of IM patients and controls in both cohorts. Both IgM and IgG levels were significantly elevated during acute IM compared to controls; (B) Vimentin-specific IgM was normalized to total IgM concentrations, which led to loss of significant differences for vimentin-specific IgM titers between IM patients and controls in cohort 1 (left panel) and cohort 2 (right panel); (C) MOG-specific IgG responses were normalized to total IgG concentrations. Despite normalization to total IgG, three IM patients in cohort 1 presented with MOG IgG reactivity above the positivity threshold. The positivity threshold, designated with a grey dotted line, was set at three standard deviations above the mean of the respective control group.