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. 2016 Feb 20;8(2):56. doi: 10.3390/v8020056

Figure 3.

Figure 3

Inhibition of PERK by GSK2606414 or by RNA interference and attenuation of eIF2α dephosphorylation by salubrinal reduced porcine circovirus 2 replication. PK-15 cells were infected with PCV2 (MOI = 1) for 36 h in the presence of GSK2606414 (GSK, 1 μM) or salubrinal (Sal, 50 μM). PK-15 cells were first transfected with PERK-specific siRNA (siPERK). Scrambled RNA was used as control. After 24 h of transfection, the cells were infected with PCV2 or mock-infected for 36 h. Whole cell lysates were subjected to Western blotting. (A–C) Representative blots of PCV2-infected cells treated with GSK2606414, siPERK and salubrinal respectively; (D,E) Inhibition of PERK by GSK2606414 or siPERK (ratio of p-PERK to t-PERK); (F) Inhibition of eIF2α dephosphorylation by salubrinal (ratio of p-eIF2α to t-eIF2α); (G–I) Effects of p-PERK inhibition by GSK2606414 or RNA interference, and inhibition of eIF2α dephosphorylation by salubrinal on viral capsid (Cap) protein expression (ratio of Cap to β-actin normalized to control cells set at 1.0, top panels) and virus titer (log TCID50/mL, bottom panels).