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. 2016 Feb 20;8(2):56. doi: 10.3390/v8020056

Figure 4.

Figure 4

Effects of GRP78 over-expression or knockdown on porcine circovirus 2 replication. PK-15 cells were first transfected with the vector expressing GRP78 or with GRP78-specific siRNA (siGRP78). Control vector pcDNA or scrambled RNA (siNC) was used as control. After 24 h of transfection, the cells were infected with PCV2 or mock-infected for indicated time points. Whole cell lysates were subjected to Western blotting for GRP78, phosphorylated eIF2α (p-eIF2α), total eIF2α (t-eIF2α), Flag tag, and viral capsid protein (Cap). (A) Representative blot showing the effects of GRP78 over-expression on target proteins; (B) Ratio of Cap to β-actin normalized to mock infection set at 1.0; (C) Virus titers expressed as log TCID50/mL; (D) Representative blot showing the effects of GRP78 silencing on target proteins; (E) Ratio of GRP78 to β-actin; (F) Ratio of Cap to β-actin normalized to control cells set at 1.0 (top panel) and virus titers expressed as log TCID50/mL (bottom panel).