Figure 1.

(A) Schematic representation of the C. albicans transporter overexpression system. The system contains the promoter and terminator regions of CDR1 and a ura3 selection marker. The SpeI cloning site in the plasmid can be used to insert the gene of interest. A S. cerevisiae PDR5 terminator is situated downstream of the SpeI site. The plasmid can be linearized by BglII digestion and integrated into the host strain DSY4684 which is devoid of three major MDR pumps of C. albicans (CDR1, CDR2, and MDR1). The host strain also has a GOF mutation in the TAC1 transcription factor as a result of which the cloned ORF is overexpressed under the native CDR1 promoter. (B) Time course fluorescence microscopy of DSY4687-5 strain (overexpressing Cdr1-GFP) grown in YEPD and YNB medium. Lower panel shows growth curve of the strain in the two mentioned medium at the time points used for microscopic analyses.