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. 2016 Mar 3;7:261. doi: 10.3389/fmicb.2016.00261

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Copyright © 2016 Banerjee, Khandelwal, Sanglard and Prasad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Confocal microscopy images of DSY4684 and DSY4687-5 strains (Upper panel) and immunoblot analyses of the PM fractions (50 μg total protein) with anti-GFP monoclonal antibody (Lower panel). (B) MIC₈₀ values of DSY4684 and DSY4687-5 cells for Fluconzole (FLC), Ketoconazole (KTZ), Miconazole (MCZ) and Rhodamine 6G (R6G). (C) Agar drug-diffusion assays of DSY4684 and DSY4687-5 cells. Susceptibility to the following azoles were tested: Fluconazole (FLC; 10 μg), Itraconazole (ITC; 1 μg), Clotrimazole (CTR; 0.5 μg), Ketoconazole (KTZ; 0.2 μg), Miconazole (MCZ; 0.2 μg) and Voriconazole (VOR; 1 μg).