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(A) Schematic diagram of the topology of Cdr1p with highlighted amino acids selected for replacement by alanine. (B) Confocal analyses of DSY4687-5 and the three constructed mutant variant strains, ANY-L529A, ANY-V532A, and ANY-C1294A. (C) Western analyses of the PM fractions (50 μg total protein) of the strains with anti-GFP monoclonal antibody.
