Figure 5.

Substrate transport assays of DSY4684, DSY4687-5 and the mutants. (A) NR accumulation in cells overexpressing Cdr1-GFP and the mutant variants as determined by Flow cytometry analyses using FL3 filter (B) R6G efflux in cells overexpressing Cdr1-GFP and the mutant variants. Assay was performed as described in the experimental section. The host DSY4684 values were subtracted from each reading. (C) [³H] FLC accumulation in cells overexpressing Cdr1-GFP and the mutant variants with respect to DSY4684 (value kept at 100%). ^*p < 0.05; ^**p ≤ 0.01.