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. 2016 Mar 3;10:47. doi: 10.3389/fncel.2016.00047

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Copyright © 2016 Mirra, Ulloa, Gutierrez-Vallejo, Martì and Soriano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Endogenous Armc10 is expressed in chicken spinal cord. (A) Structure and domains of the two different isoforms of chicken Armc10 protein predicted by in silico analysis. They differ in the N-terminal region, containing a mitochondrial targeting peptide, an N-terminal transmembrane region, and a predicted cleavage site. The C-terminal domain consists of up to six Arm-like tandem repeats. (B) Both Armc10 isoforms share a strong sequence homology with mouse Armc10. (C) HH stage 12, 19, and 24 showing expression of chick Armc10. (D) Efficiency of Armc10 gene silencing using shRNA in chicken neural tube, as measured with RT-qPCR. Armc10 mRNA expression was calculated relative to that of control shRNA (shControl; error bars indicate the SD).