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. 2016 Mar 3;10:47. doi: 10.3389/fncel.2016.00047

Figure 9.

Figure 9

Endogenous Armc10 silencing inhibits progenitor proliferation but does not affect neural maturation. (A–F) Representative transverse sections of neural tubes from embryos electroporated at HH stage 12 with shControl and shArmc10 plasmids and analyzed at 48hpe with the indicated immunostaining. No changes in the distribution of shArmc10 expressing cells (D) or in the percentage of HuC/D/GFP and Sox2/GFP-positive cells (F) are observed (anti-HuC/D, blue; anti-Sox2, red). (G,H) The HuC/D+ areas corresponding to the MZ (formed by the differentiating neurons) were defined using ImageJ processing (G). The areas measured for the electroporated side (EP) were standardized to their contralateral controls (CNTRL) and are presented as ratios of the area of MZ (HuC/D+Area); the widths of the Tuj-1-marked region for the electroporated side were standardized to their contralateral controls and are presented as ratios of the size of MZ (Tuj+ Size) (H). (I) Histogram showing the percentage of electroporated cells (GFP+) positive for Tuj-1. Data represent the mean ± s.e.m. (*p < 0.05).