Figure 4. dFoxO is required for the wing patterning functions of Wg signaling.

Light micrographs of Drosophila adult wings are shown. Compared with the ptc-Gal4 control (a, the right panel shows high magnification of the boxed area containing ACV in the left panel), expression of Arm induces ectopic bristles (indicated by the red arrow head) and loss-of-ACV phenotypes (b), which remain unaffected by the expression of LacZ (c), but are largely suppressed by RNAi-mediated knocking-down of dfoxo (d) or heterozygous mutation of dfoxo^Δ94 (e). Compared with the sd-Gal4 control (f, the right panel shows high magnification of the boxed area containing anterior-distal wing margin in the left panel), expression of Arm induces ectopic bristles near the wing margin (g, indicated by red arrow heads), which remains unaffected by the expression of LacZ (h), but is suppressed by knocking-down of dfoxo (i) or heterozygous mutation of dfoxo^Δ94 (j). knocking-down of wg by ptc-Gal4 generates a mild wing margin notch phenotype between the L3 and L4 veins (k, indicated by the red box and amplified in the right panel), which is rescued by the expression of dFoxO (l), but is enhanced by heterozygous mutation of dfoxo²¹ (m) or dfoxo²⁵ (n). Sample numbers: a, 226; b, 201; c, 108; d, 197; e, 160; f,168; g, 166; h, 146; i, 130; j, 178; k,160; l, 62; m, 174; n, 226. Scale bars: 100 μm in (a–n) and 50 μm in high magnification figures.