Figure 5. dFoxO is required for Wg target gene activation.

Light micrographs of Drosophila 3^rd instar wing discs (a–f) and salivary glands (g–l) with X-Gal staining are shown. Compared with the ptc-Gal4 control (a,g), ectopic Arm-induced wf-LacZ expression in the wing disc (b, indicated by black arrow heads) or salivary gland (h) remains unaffected by the expression of GFP (c and i), but is significantly suppressed by knocking-down of dfoxo (d,j), or heterozygous mutation of dfoxo²⁵ (e,k) or dfoxo^Δ94 (f,l). Scale bars: 100 μm in (a–f) and 200 μm in (g–l).