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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Integr Biol (Camb). 2016 Jan 25;8(2):243–252. doi: 10.1039/c5ib00225g

Figure 6. Diphenyleineideom (DPI) inhibits PMA induced neutrophil activation.

Figure 6

A, The mean fluorescence intensity of cells treated with 1μM PMA for 30 minutes with or without the addition of varying concentrations of DPI inhibitor. Fluorescence is measured by dihydrorhodamine-123 (DHR) signal relative to positive control cells activated with 1μM of PMA without inhibitor. There is a statistically significant decrease in formation of reactive oxygen species measured by DHR signal as determined by oneway ANOVA (p-value < 0.001). B, The percent of NETs produced in 3 hours after activation with 1μM PMA in the presence or absence of the indicated concentration of DPI. All data was repeated in duplicate or in triplicate at least three times.