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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Integr Biol (Camb). 2016 Jan 25;8(2):243–252. doi: 10.1039/c5ib00225g

Figure 7. Pseudomonas aeruginosa induced NETosis.

Figure 7

A-B, Representative images of NETosis with Hoechst staining of (A) untreated neutrophils and (B) 1μM PMA treated neutrophils after 3 hour incubations. C-E, Representative images of NETs produced following stimulation with overnight cultures of P. aeruginosa for 8 hours. (C) Hoechst staining of neutrophils cultured with P. aeruginosa. (D) mCherry labeled P. aeruginosa added to neutrophils. (E) Overlay of blue Hoechst staining with red mCherry labeled P. aeruginosa. Arrows indicate localization of bacteria to NETs. All data was repeated in duplicate or in triplicate at least three times. White bar indicates 10 μm.