Fig. 3.

The effects of signal peptide hydrophobicity on full-length hu5D5 production. For all the constructs, light chain was fused to ssSTII0.3 and heavy chain was fused to ssSTII1, ssDsbA1, ssPhoA1, ssMalE1, ssSTII1 S-13L, ssSTII1 S-13I, ssDsbA1 L-9S, or ssDsbA L-9I. Top panel the non-reduced soluble protein samples from the cell lysates were separated by electrophoresis followed by Western blot analysis. Heavy chain-containing species are marked based on the apparent molecular weight. Middle panel Western blot of soluble heavy chain in the periplasm. The periplasmic protein samples were reduced with 0.2 M DTT. Bottom panel Western blot of reduced total heavy chain in the whole cell lysates. All the blots were probed with the HRP-conjugated anti-Fc antibody