Table 3.
The effects of signal peptide hydrophobicity on hu5D5 heavy chain processing
| Signal peptide | ΔHydro | Mature HC % | Signal peptide | ΔHydro | Mature HC % | |
|---|---|---|---|---|---|---|
| HC only | ssSTII1 | – | 29 | ssDsbA1 | – | 84 |
| ssSTII1 S-13L | +1.24 | 70 | ssDsbA1 L-9S | −1.24 | 38 | |
| ssSTII1 S-13I | +1.56 | 58 | ssDsbA1 L-9Q | −1.91 | 20 | |
| ssSTII1 S-13Y | +0.44 | 22 | ssDsbA1 L-9A | −0.44 | 60 | |
| ssSTII1 A-18L A-14L S-13L | +2.12 | 65 | ssDsbA1 L-9I | +0.32 | 82 | |
| LC and HC co-expressed | ssSTII1 | – | 45 | ssDsbA | – | 86 |
| ssSTII S-13L | +1.24 | 67 | ssDsbA1 L-9S | −1.24 | 39 | |
| ssSTII S-13I | +1.56 | 66 | ssDsbA1 L-9I | +0.32 | 81 |
ΔHydro represents the change in total hydrophobicity resulting from the amino acid variants