Table 1.
Product distributions of wild-type TXS and important variants
| Target | Mutation | Activity* | T† | T1 | CM | V | V1 | V2 |
| TXS | 100.0 | 93.2 | 4.7 | N.D. | 0.8 | 0.2 | 1.1 | |
| V584 | V584M | 92.3 | 13.8 | 0.6 | N.D. | 83.4 | 0.8 | 1.4 |
| V584 | V584L | 92.1 | 13.8 | 0.6 | N.D. | 83.4 | 0.8 | 1.4 |
| S587 | S587A | 21.8 | 8.9 | N.D. | 58.9 | 32.2 | N.D. | N.D. |
| W753 | W753H | 51.3 | N.D. | N.D. | 100.0 | N.D. | N.D. | N.D. |
| Y841 | Y841F | 41.3 | N.D. | N.D. | 56.4 | 43.6 | N.D. | N.D. |
*
Enzyme activities of mutants (in percentage) in comparison with TXS (=100%). The activities were determined by peak integration of the enzyme products in relation to the peak area of the internal standard α-humulene (Materials and Methods).
†
Compound numbers CM–T1 refer to compound numbers in Fig. 1 and SI Appendix, Fig. S8A. The tabulated values represent the product distributions in percentage for TXS and each mutant (sum = 100%). N.D., not detectable.