Fig. 4.

[¹⁸F]FDG does not allow identification of A. fumigatus infection. (A) Sagittal MIP, MRI, and PET/MRI images of PBS-treated-, S. pneumoniae-, and A. fumigatus-infected mice imaged with [¹⁸F]FDG (3, 24, and 48 h after infection). All animals, including the PBS-treated mice, presented a highly elevated uptake of [¹⁸F]FDG within the lungs 24 and 48 h after the infection with A. fumigatus or S. pneumoniae. (B) Quantification of the in vivo PET images showed no significant differences in the uptake of [¹⁸F]FDG in the lungs of A. fumigatus-infected animals compared with the lungs of the control groups 24 and 48 h after infection. (C) The ex vivo biodistribution confirmed the in vivo [¹⁸F]FDG-PET results with no significant differences in the uptake of the lungs of A. fumigatus-infected animals compared with the lungs of control animals 48 hpi. Black bars, PBS-treated controls (n = 10); blue bars, S. pneumoniae-infected mice (n = 5); red bars, A. fumigatus-infected mice (n = 5). Data are expressed as the average ± SD (%ID/cc: PET; %ID/g: ex vivo biodistribution), one-way ANOVA, post hoc Tukey–Kramer corrected for multiple comparisons, *P < 0.05.