Fig. S2.

[⁶⁴Cu]DOTA-JF5 as disease-specific PET-tracer for A. fumigatus lung infection. Sagittal MIP, MRI, and fused PET/MRI images of PBS-treated-, S. pneumoniae-, Y. enterocolitica-, and A. fumigatus-infected mice injected with [⁶⁴Cu]DOTA-JF5 (3, 24, and 48 h after infection). (A) Tracer injection demonstrates highly specific accumulation in A. fumigatus-infected lung tissue compared with bacterial-infected or sham-treated animals already 24 h after infection and injection of the tracer. (B) Quantification of the in vivo PET images showed a significantly higher uptake of [⁶⁴Cu]DOTA-JF5 in the lungs of A. fumigatus-infected animals compared with the lungs of the control groups 24 and 48 h after infection. (black bars, PBS-treated controls; blue bars, S. pneumoniae-infected mice; orange bars, Y. enterocolitica-infected mice; red bars, A. fumigatus-infected mice). Data are expressed as the average ± SD (%ID/cc: PET; %ID/g: ex vivo biodistribution), one-way ANOVA, post hoc Tukey corrected for multiple comparisons, *P < 0.05.