Fig. S4.

Kal-7 enhancement of synaptic AMPAR function requires Cdc42. (A and B) Scatterplots showing amplitudes of AMPAR- and NMDAR-eEPSCs of control and transfected neurons in cultured hippocampal rat slices. Filled circles represent mean ± SEM. (A) Distributions showing that coexpression of Kal-7 and a Cdc42-shRNA for 6 d had no effect on AMPAR-eEPSC amplitude (Upper; n = 7 pairs) or NMDAR-eEPSC amplitude (Lower; n = 6 pairs). (B) Expression of the Cdc42-shRNA for 6 d had no effect on AMPAR-eEPSC amplitude (Upper; n = 8 pairs) or NMDAR-eEPSC amplitude (Lower; n = 7 pairs). (Insets) Sample current traces from a control neuron (black) and a transfected neuron (green). (Scale bars: 20 ms for AMPA, 50 ms for NMDA, 20 pA.) (C) Bar graphs normalized to control comparing mean ± SEM AMPAR- and NMDAR-eEPSC data in A and B with that shown in Fig. 1B (gray bar).