Fig. S6.

Kal-miR and Kal-7 (T95A) coexpression blocks CA-CaMKII enhancement of synaptic AMPAR function. (A–C) Scatterplots showing amplitudes of AMPAR- and NMDAR-eEPSCs for single pairs of control and transfected neurons (open circles). Filled circles indicate mean ± SEM. (A) Distributions showing that CA-CaMKII OE for 2–3 d increases AMPAR-eEPSC amplitude (Upper; n = 12 pairs; *P = 0.01), but not NMDAR-eEPSC amplitude (Lower; n = 10 pairs). (B) Coexpression of Kal-miR, Kal-7 (T95A), and CA-CaMKII had no effect on AMPAR-eEPSC amplitude (Upper; n = 9 pairs) or NMDAR-eEPSC amplitude (Lower; n = 6 pairs). (C) Coexpression of Kal-miR, Kal-7, and CA-CaMKII increased AMPAR-eEPSC amplitude (Upper; n = 8 pairs; *P = 0.02), but not NMDAR-eEPSC amplitude (Lower; n = 7 pairs). (D) Bar graphs normalized to control summarizing mean ± SEM AMPAR- and NMDAR-eEPSC data in A–C. (Insets) Sample current traces from control (black) and transfected (green) neurons. (Scale bars: 20 ms for AMPA, 50 ms for NMDA, 20 pA.)