Fig. 2.

Activation of RNase L during SINV infection of A549 cells requires OAS3 expression. (A) WT A549 and KO cells were infected with SINV (MOI = 1 pfu per cell), and at 24 hpi cells were lysed and RNA integrity was assessed. The positions of 18S and 28S rRNA are indicated. (B) Cells were infected with SINV (MOI = 1 pfu per cell), and at 24 hpi intracellular levels of 2-5A were quantified by FRET assay. (C) Cells were infected with SINV (MOI = 1 pfu per cell), and at the indicated time points infectious virus in the supernatant was titered by plaque assay on Vero cells. The data are from three biological replicates and are expressed as means ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.