Fig. 4.

Activation of RNase L during IAVΔNS1 infection of A549 cells requires OAS3 expression. (A) WT A549 and KO cells were infected with IAVΔNS1 (MOI = 10), and at 24 hpi RNA integrity was assessed. (B) Cells were infected with IAVΔNS1 (MOI = 1 pfu per cell), and at 24 hpi 2-5A intracellular levels were quantified by FRET assay. (C) Cells were infected with IAVΔNS1 (MOI = 1 pfu per cell), and at 24 hpi infectious virus in the supernatants was titered by plaque assays. The data are from three biological replicates and are expressed as means ± SD; **P < 0.01.