Fig. 5.

Activation of RNase L during VACVΔE3L infection of A549 cells requires OAS3 expression. (A) WT A549 cells and KO cells were infected with VACVΔE3L (MOI = 10 pfu per cell), and at 24 hpi RNA integrity was assessed. (B) Cells were infected with VACVΔE3L (MOI = 1 pfu per cell), and at 24 hpi 2-5A intracellular levels were quantified by FRET assay. (C) Cells were infected with VACVΔE3L (MOI = 1 pfu per cell), and at 24 hpi infectious virus in the supernatants was titered by plaque assays on BHK21 cells. The data are from three biological replicates and are expressed as means ± SD; **P < 0.01.