Fig. 6.

Activation of RNase L during WNV infection of HT1080 cells requires OAS3 expression. (A) OAS1-, OAS2-, OAS3-, and RNase L-KO HT1080 cells were mock-treated or were treated with IFN-α (2,000 U/mL) overnight. Proteins were analyzed by immunoblotting with antibodies against OAS1, OAS2, OAS3, and RNase L. (B) Cells were infected with WNV (MOI = 20 pfu per cell), and at 24 hpi RNA integrity was assessed. (C) Cells were infected WNV (MOI = 1 pfu per cell), and at the indicated time points infectious virus from the supernatant was titered by plaque assay. The data are from three biological replicates and are expressed as means ± SD; *P < 0.05, ***P < 0.001.