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. 2016 Feb 8;113(8):2241–2246. doi: 10.1073/pnas.1519657113

Fig. 7.

Fig. 7.

OAS3 binds to dsRNA with higher affinity than do OAS1 or OAS2 in intact cells. (A) A549 cells were transfected with empty vector (mock) or plasmids encoding Flag-tagged OAS1 p42, OAS1 p46, OAS2, or OAS3 and then were transfected with pIC-biotin. Streptavidin bead pull downs or proteins were carried out and were analyzed by immunoblotting with anti-Flag antibody. The blot was exposed for 5 or 20 min. Immunoblots without pull down show input levels of OAS proteins and GAPDH loading and transfer control. The additional, faster-migrating band in the p46 lanes is most likely a breakdown product of p46. (B) A549 cells were treated with IFN-β (1,000 U/mL) and then were transfected with pIC-biotin. Streptavidin bead pull down of pIC-biotin bound proteins was carried out and probed by immunoblotting with antibodies against OAS1, OAS2, and OAS3. Input levels of proteins are also shown. Thirty-fold more protein was used for pull down compared with input protein on the immunoblot.